Mass blood survey for malaria: pooling and realtime…

Background: Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in bothlow-and high-transmission settings and represents an important reservoir of infection thatneeds to be targeted if malaria elimination is to succeed. Methods: Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son,an area of endemic but low-transmission malaria in the north-west of Thailand. Themicroscopist at the local malaria clinic did not detect any infections. Pools of four sampleswere screened by real-time PCR; individual members of all of the positive pools were thenre-examined by expert microscopy and by a second species-specific PCR reaction. Results: Eight subjects were found to be positive by both PCR and expert microscopy and one wasfound to be positive by PCR alone

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Mass blood survey for malaria: pooling and realtime…