Real-time PCR diagnosis of Plasmodium vivax among blood donors

Background: When selecting blood donors in transfusion centres, one important problem is to identify,during screening, individuals with infectious diseases that can be transmitted by blood, suchas malaria, especially when the parasite densities are very low. This problem is particularlysevere in endemic areas, such as the Brazilian Amazon. In the present study, moleculardiagnostic (real-time PCR) of Plasmodium vivax was used to identify blood donors infectedwith malaria parasites. Methods: Samples from 595 blood donors were collected in seven haemotherapy centres in northernBrazil located in areas at risk for malaria transmission, and the analyses were performed byreal-time PCR with TaqMan probes on 7500 Real-Time PCR Systems, to genotype themitochondrial DNA region specific to P.

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Real-time PCR diagnosis of Plasmodium vivax
among blood donors