Latest posts by Tropical Med and Intl Health (see all)
- Quality of surgical care in hospitals providing internship training in Kenya - Oct. 28, 2014
- Nodding syndrome since 2012: recent progress, challenges and recommendations for future… - Oct. 28, 2014
- Sex differences in nutritional status of HIV-exposed children in Rwanda: a longitudinal study - Oct. 27, 2014
- Peritoneal dialysis vs. haemodialysis in the management of paediatric acute kidney injury in… - Oct. 27, 2014
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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