Latest posts by Tropical Med and Intl Health (see all)
- Health service delivery models for the provision of antiretroviral therapy in sub-Saharan… - Jul. 28, 2014
- Typhoid fever in Fiji: a reversible plague? - Jul. 25, 2014
- Assessing the impact of a school-based latrine cleaning and handwashing program on pupil… - Jul. 24, 2014
- Severe events in the first 6 months of life in a cohort of HIV-unexposed infants from South… - Jul. 23, 2014
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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