Latest posts by Tropical Med and Intl Health (see all)
- Trends in stroke admissions to a Tanzanian hospital over four decades: A retrospective audit - May. 15, 2015
- Household Coping Strategies for Delivery and Related Health Care Cost: Findings from Rural… - May. 15, 2015
- Schistosoma mansoni and HIV infection in a Ugandan population with high HIV and helminth… - May. 15, 2015
- Cost per patient of treatment for rifampicin-resistant tuberculosis in a community-based… - May. 15, 2015
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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