Latest posts by Tropical Med and Intl Health (see all)
- Diarrhea in Children under-five years of age in South Africa (1997-2014) - Jun. 20, 2016
- Infant oral mutilation in East Africa ‒ therapeutic and ritual grounds - Jun. 17, 2016
- Hypoxaemia in Mozambican children < 5 years of age admitted to hospital with clinical severe… - Jun. 17, 2016
- Seroepidemiology: an underused tool for designing and monitoring vaccination programs in low… - Jun. 16, 2016
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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