Latest posts by Tropical Med and Intl Health (see all)
- Prospective study of cigarette and alcohol use among adults in a rural Uganda - Dec. 15, 2014
- Compounding diagnostic delays: a qualitative study of point-of-care testing in South Africa - Dec. 12, 2014
- The relationship between HIV and Prevalence of Disabilities in sub-Saharan Africa: Systematic… - Dec. 12, 2014
- Explaining low rates of sustained use of Siphon water filter: Evidence from follow-up of a… - Dec. 12, 2014
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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