Latest posts by Tropical Med and Intl Health (see all)
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- Improved detection of Zika virus RNA in human and animal specimens by a novel, highly sensitive… - Feb. 18, 2017
- Placental pathology in HIV infection at term: a comparison with HIV-uninfected women - Feb. 18, 2017
- Human-to-human transmission of Brucella – a systematic review - Feb. 14, 2017
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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