Latest posts by Tropical Med and Intl Health (see all)
- Antibiotic prescribing practice for acute, uncomplicated respiratory tract infections in… - Apr. 22, 2014
- Distance to emergency obstetric services and early neonatal mortality in Ethiopia - Apr. 22, 2014
- Soil-transmitted helminths in southern highland Rwanda: associated factors and effectiveness of… - Apr. 21, 2014
- Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of… - Apr. 18, 2014
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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