Latest posts by Tropical Med and Intl Health (see all)
- Challenges of bacterial meningitis case management in Ethiopia - May. 04, 2016
- Parental migration and children’s timely measles vaccination in rural China: a cross-sectional… - May. 03, 2016
- Economic burden of gastrointestinal cancer under the protection of the New Rural Cooperative… - May. 02, 2016
- Drug resistance mutations after the first 12 months on antiretroviral therapy and determinants… - May. 02, 2016
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
See original article here: