Latest posts by Tropical Med and Intl Health (see all)
- Cost of neurocysticercosis patients treated in two referral hospitals in Mexico City, Mexico - Feb. 28, 2015
- Women’s perceptions of the quality of emergency obstetric care in a referral hospital in rural… - Feb. 28, 2015
- Availability and price of malaria rapid diagnostic tests in the public and private health… - Feb. 27, 2015
- Spatial analysis of under-5 mortality and potential risk factors in the Basse Health and… - Feb. 27, 2015
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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