Latest posts by Tropical Med and Intl Health (see all)
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- Factors associated with death from dengue in the state of Minas Gerais, Brazil: historical… - Oct. 24, 2014
- Hepatitis C virus acquisition among Egyptians: analysis of a 10-year surveillance of acute… - Oct. 22, 2014
- Peppermint (Mentha piperita) and albendazole against anisakiasis in an animal model - Oct. 20, 2014
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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