Latest posts by Tropical Med and Intl Health (see all)
- Infant twin mortality and hospitalisations after the perinatal period – a prospective cohort… - Sep. 22, 2014
- Socio-economic and environmental effects influencing the development of leprosy in Bahia,… - Sep. 22, 2014
- Tropical Medicine & International Health October 2014 - Sep. 17, 2014
- Retention and risk factors for attrition among adults in antiretroviral treatment programmes in… - Sep. 17, 2014
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
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