Latest posts by Tropical Med and Intl Health (see all)
- Seasonal and gestation stage associated differences in aflatoxin exposure in pregnant Gambian… - Dec. 21, 2013
- Treatment outcomes in a cohort of Palestine refugees with diabetes mellitus followed through… - Dec. 17, 2013
- Dengue epidemics and adverse obstetrical outcomes in French Guiana: a semi-ecological study - Dec. 17, 2013
- Measuring disparities in sanitation access: does the measure matter? - Dec. 17, 2013
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh). Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S. haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S. haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
See original article here: