Detection of Serotype-Specific Antibodies to the Four Dengue Viruses Using an Immune Complex…

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PLoSNTDs

PLoSNTDs

PLOS Neglected Tropical Diseases is the first open-access journal devoted to the world's most neglected tropical diseases (NTDs), such as elephantiasis, river blindness, leprosy, hookworm, schistosomiasis, and African sleeping sickness. Links to PLoSNTD content is shared via GHHub using the Creative Commons. http://www.plosntds.org/

by Petra Emmerich, Angela Mika, Herbert Schmitz Background Dengue virus (DENV) infections are preferentially diagnosed by detection of specific IgM antibodies, DENV NS1 antigen assays or by amplification of viral RNA in serum samples of the patients. The type-specific immunity to the four worldwide circulating DENV serotypes can be determined by neutralization assays. An alternative to the complicated neutralization assays would be helpful to study the serotype-specific immune response in people in DENV hyperendemic areas but also in subjects upon DENV vaccination. Methods In consecutive samples of patients with DENV-1- 4 infection type-specific antibodies were detected using an immune complex binding (ICB) ELISA. During incubation of serum samples and enzyme- labeled recombinant envelope domain III (EDIII) antigens immune complexes (ICs) are formed, which are simultaneously bound to a solid phase coated with an Fc–receptor (CD32)

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Detection of Serotype-Specific Antibodies to the Four Dengue Viruses Using an Immune Complex…